Hundreds of museums and galleries in the UK and Europe have signed up to take part in the project, which runs from 24 to 30 March on the social networking site.
As well as using #MuseumWeek in tweets during the week, there will be a different theme every day: #DayInTheLife (Monday); #MuseumMastermind (Tuesday); #MuseumMemories (Wednesday); #BehindtTheArt (Thursday); #AskTheCurator (Friday); #MuseumSelfies (Saturday); and #GetCreative (Sunday).
Some organisations will run their own projects during the week. Historic Royal Palaces will share a day in the life of Henry VIII and a Twitter Q&A on the former monarch, on 24 March. The Natural History Museum and the Victoria and Albert Museum will promote #MuseumSelfies at their respective late-night events.
Other UK organisations taking part include London's Science Museum and British Museum, and Tate; the Roald Dahl Museum in Buckinghamshire; the Pencil Museum in Cumbria; Tenby Museum and Art Gallery in Pembrokeshire; and the Time and Tide Museum in Great Yarmouth.
A full list of UK and European museums signed up to the initiative can be viewed on Twitter. Museums can register in the run up to the event or take part during the week by using the hashtag.
The Design Museum in London said it will offer followers behind-the-scenes perspectives, puzzles, giveaways and a Q&A session with the Designs of the Year 2014 exhibition curator.
Tate, which is the most followed museum on Twitter in Europe with more than one million followers, has previously run a live tour of its Roy Lichtenstein retrospective on Twitter.
Its digital communications manager Jesse Ringham said: “Tate's presence on Twitter is crucial to engaging our global audiences in ongoing conversations about art and creativity. We’re delighted to be joining this international initiative alongside large and small museums and galleries from the UK and beyond."
Mar Dixon, who runs the @CultureThemes project and helps museums improve their social media presence, is encouraging more museums to take part.
On a recent blog post she said the objective of the campaign is to encourage more museums to use Twitter, and reach new audiences and advocates: “I am really proud that Twitter has seen the potential with the museum sector – Go us!”
The April edition of Museum Practice will look at social media. Click here to submit a case study.